3. (4 pts) Calculate the ratio of [ONOʻ]/[HONO] at pH 4.5 for nitrous acid, HONO, PK, = 3.25. 4. (6 pts) Many mutant f
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3. (4 pts) Calculate the ratio of [ONOʻ]/[HONO] at pH 4.5 for nitrous acid, HONO, PK, = 3.25. 4. (6 pts) Many mutant f
4. (6 pts) Many mutant forms of hemoglobin are known to exist. One such mutation known as Yakima has an Asp à His mutation in the beta chain. This mutation disrupts an H-bond that typically stabilizes the T-state. What effect, if any, would you predict this mutation to have on the K, of O,? Draw an o, binding curve for both WT and mutant Hb to support your answer.