(b) In an experiment using HPLC to determine the concentration of paracetamol in a trademark flu remedy, a stock solutio

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B In An Experiment Using Hplc To Determine The Concentration Of Paracetamol In A Trademark Flu Remedy A Stock Solutio 1 (65.95 KiB) Viewed 7 times

(b) In an experiment using HPLC to determine the concentration of paracetamol in a trademark flu remedy, a stock solution of pure paracetamol with a concentration of 0.10 mg/cm3 was diluted by adding 2.5 cm3 to a 50 cm3 volumetric flask and making up to the mark with water. This test solution was then injected into the instrument and a chromatogram produced which contained one peak with a retention time of 1.212 minutes and a peak area of 880.45 mAUS. A sample of the flu remedy solution was then injected into the instrument and a chromatogram was obtained with three main peaks as detailed on the next page. Calculate the concentration of paracetamol in the solution. Peak # Retention time (min) 1 2 3 4 0.932 1.207 2.125 3.354 Peak Area (mAUS) 452.8 1081.3 623.1 45.7