b) A peptide is hydrolysed to form a solution containing a mixture of amino acids. This mixture is then analysed by sili

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B A Peptide Is Hydrolysed To Form A Solution Containing A Mixture Of Amino Acids This Mixture Is Then Analysed By Sili 1 (107.07 KiB) Viewed 14 times

b) A peptide is hydrolysed to form a solution containing a mixture of amino acids. This mixture is then analysed by silica gel thin- layer chromatography (TLC) using a toxic solvent. The individual amino acids are identified from their Rr values. Part of the practical procedure is given below: 1. Wearing nitrile gloves to hold a TLC plate, draw a pencil line 1.5 cm from the bottom of the plate. 2. Use a capillary tube to apply a very small drop of the solution of amino acids to the mid-point of the pencil line. 3. Allow the spot to dry completely. 4. In the developing tank, add the TLC solvent to a depth of not more than 1 cm. 5. Place your TLC plate in the developing tank. 6. Allow the TLC solvent to rise up the plate to the top. 7. Remove the plate and quickly mark the position of the solvent front with a pencil. 8. Allow the plate to dry in a fume cupboard. For each stage of the procedure presented in bold, consider whether it is essential or not for the success of the practical, and justify your answer for each. i) ii) Stage 1 - Wearing nitrile gloves to hold a TLC plate Stage 4 - Add the TLC solvent to a depth of not more than 1 cm3 Stage 6 - Allow the TLC solvent to rise up the plate to the top Stage 8 - Allow the plate to dry in a fume cupboard · iii) iv) (4 marks)