Chromatography Scaleup It is desired to scale up the throughput by a factor of 150 for a linear gra- dient ion exchangé

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Chromatography Scaleup It Is Desired To Scale Up The Throughput By A Factor Of 150 For A Linear Gra Dient Ion Exchange 1 (258.54 KiB) Viewed 13 times

Chromatography Scaleup It is desired to scale up the throughput by a factor of 150 for a linear gra- dient ion exchangé chromatography of a product protein from the laboratory to the plant. The condi- tions for the laboratory chromatography are the fol- lowing: 1.0 cm bed diameter (D) x 20 cm bed height, 20. um particle size, and 30 cm/h superficial velocity. The particle size of the same type of ion exchange resin available for the plant operation is 40 um. Two columns are available in the plant: one column 14.0 cm diameter (D) x 50 cm high, and another column 18.0 cm diameter (ID) x 50 cm high. To keep the resolution for the chromatography constant in the plant, which column should beņused? For this column, what should be the resin bed height and the superficial velocity and what do you esti- mate the pressure drop to be? The viscosity of the mobile phase is 1.0 cp, and the void fraction for resin in the plant column is 0.33.