A newly appointed biochemical engineer was tasked with inoculum preparation and scale up of a culture of a sensitive bac

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A Newly Appointed Biochemical Engineer Was Tasked With Inoculum Preparation And Scale Up Of A Culture Of A Sensitive Bac 1 (65.95 KiB) Viewed 9 times

A newly appointed biochemical engineer was tasked with inoculum preparation and scale up of a culture of a sensitive bacterium strain. They undertook the following operating procedure: Step 1: They prepared the working culture of the bacterium on an agar slant, and waited for 1 day Step 2: They added saline and glass beads to the slant, and waited for 1 day Step 3: They transferred the culture to a shake flask preloaded with fresh agar, and waited for 1 day Step 4: They transferred the culture to a seed fermenter and waited for 1 day Step 5: They transferred the fermenter contents to the production fermenter (a) What was the purpose of the 1 day waiting time between steps? Use an appropriate sketch to support your explanation. [4 marks) (b) Tests carried out on the production fermenter indicated that the cell mass concentration was far below the level expected. Review the engineer's operating procedure and identify three possible reasons for this. [6 marks] (c) Consider the relevance of the five pillars of GMP to the scenario detailed in this question and propose one specific improvement for each pillar. [8 marks) (d) Out of the improvements you proposed in part (c), which do you think is the most important? Justify your choice. [2 marks]