You are working to characterize a novel protein in mice. Characterization of the mRNA
expression pattern revealed that the gene is highly expressed in brain, muscle, liver,
and pancreas. To determine the size of the corresponding protein, you make protein
extracts from each of the tissues. Using an antibody that recognizes the C-terminal
portion of the protein, you perform an analysis that allows you to visualize the extracted protein. Interestingly, you can detect the protein in brain, muscle, and liver cells, but not the pancreas. Assuming your extracts and reagents are good, and there is no human error, what is the most likely explanation for this result?
a. Transcription levels in the pancreas are too low.
b. Translation levels in the pancreas are too low.
c. Alternative splicing in the pancreas leads to protein degradation.
d. Alternative splicing in the pancreas yields a protein missing the portion of the
protein to which the antibody was raised.
You are working to characterize a novel protein in mice. Characterization of the mRNA expression pattern revealed that t
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You are working to characterize a novel protein in mice. Characterization of the mRNA expression pattern revealed that t
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