Laboratory 6 DNA purification Pretreatment: Maceration and cell lysis • Protein denaturation • Phenol:chloroform or chlo

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Laboratory 6 DNA purification Pretreatment: Maceration and cell lysis • Protein denaturation • Phenol:chloroform or chlo

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Laboratory 6
DNA purification
Pretreatment: Maceration and cell lysis
• Protein denaturation
• Phenol:chloroform or chloroform:isoamyl extraction for removal of
most proteins and carbohydrates
• DNA precipitation
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