Question 4 a Describe an experiment by means of which you can demonstrate that after treatment of human oviduct cells wi

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Question 4 A Describe An Experiment By Means Of Which You Can Demonstrate That After Treatment Of Human Oviduct Cells Wi 1 (58.51 KiB) Viewed 45 times

Question 4 a Describe an experiment by means of which you can demonstrate that after treatment of human oviduct cells with estrogen, a full-length copy of the ovalbumin mRNA is synthesized (2155 bp linear mRNA) 131 b. There are two versions of the thyroid hormone receptor produced in human cells. These two proteins differ in size and are produced in different relative amounts in tissue A and tissue B. How would you experimentally demonstrate that the difference between A and B is determined by alternative splicing? 141 c. You would like to study the different proteins that are synthesized after induction with a hormone. a Describe the type of information you can obtain from 2D electrophoresis. 131 6. How can you use the protein spots, unique to cells stimulated with hormone, to obtain information of their identity? 191 Questions The pre-mRNA encoding the transposase enzyme contains 3 introns and 4 exons in germline tissues, all three introns are removed and functional transposase enzyme produced. In contrast, intron 3 is not removed in somatic cells, hence non-functional transposase is produced (Figure 1). As a young scientist, you have been assigned to investigate the molecular mechanism of alternative splicing of transposase in germline and somatic cells. an DNA NA MA fig Contents to tanto tiene AMMA AMMAS Figure 1: Schematic presentation of transposase pre-mRNA processing in somatic and germline cells. a) Provide a model (schematic presentation) to serve as a basis for future studies to elucidate the mechanism underlying the alternative splicing event of transposase pre-mRNA in somatic cells. Take into account that the 5'- and 3' ends of both transcripts are identical. [5] b) Name and explain (in no more than 3 sentences) a technique suitable to experimentally evaluate your model. Provide a suitable negative control in your answer. 131 c) Explain how the spliced mRNA in somatic cells will be recognized and targeted for degradation. [2] Question 151 Cellular compartmentalization is essential for the correct processing, trafficking and degradation of bioactive molecules. Explain the latter statement using the process of mRNA degradation as example.