10 84 A Od 260 4 2 0 45 50 55 60 65 70 75 80 85 90 95 100 Temperature C A B C Explain What Od Stands For 1 (27.31 KiB) Viewed 32 times
10 84 A Od 260 4 2 0 45 50 55 60 65 70 75 80 85 90 95 100 Temperature C A B C Explain What Od Stands For 2 (27.31 KiB) Viewed 32 times
10- 84 A (OD 260) 4- 2- 0+ 45 50 55 60 65 70 75 80 85 90 95 100 Temperature (°C) (a) (b) (c) Explain what OD stands for. (1) What are the characteristics of the light used to measure the absorbance? (1) Explain what happens to the DNA between 75°C and 85°C. (3) Explain what happens to the DNA as the solution is cooled again. (3) If you were to heat a solution of DNA rich in A-T bonds, what difference might you observe and why? (2) (d) (e)
10- 84 A (OD 260) 4- 2- 0+ 45 50 55 60 65 70 75 80 85 90 95 100 Temperature (°C) (a) (b) (c) Explain what OD stands for. (1) What are the characteristics of the light used to measure the absorbance? (1) Explain what happens to the DNA between 75°C and 85°C. (3) Explain what happens to the DNA as the solution is cooled again. (3) If you were to heat a solution of DNA rich in A-T bonds, what difference might you observe and why? (2) (d) (e)
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