Question 2 The Bradford Method Described In The Background Section Was Used To Determine Protein Concentrations Of Known 1 (32.78 KiB) Viewed 110 times
Question 2 The Bradford Method Described In The Background Section Was Used To Determine Protein Concentrations Of Known 2 (57.1 KiB) Viewed 110 times
Question 2 The Bradford Method Described In The Background Section Was Used To Determine Protein Concentrations Of Known 3 (32.47 KiB) Viewed 110 times
QUESTION 2 The Bradford method described in the Background section was used to determine protein concentrations of known and unknown samples The following results was obtained: Table 1: Absorbance at 595nm obtained for different concentrations of protein standards using the Bradford method Protein (mg/ml) 0.00 0.125 0.25 0.50 0.75 1.00 1.50 2.00 Unknown 0 Abs. BSA 0.130 0.350 0.620 0.870 1.600 1.120 1,400 0.930 0 Abs, Big 0.080 0.230 0.460 0.520 0.700 0.410 0.510
1.8 1.7 1.6 1.5 Abs. BSA Abs, Big 14 13 12 1.1 1.0 Absorbancy (595nm) 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0.0 0.00 0.25 0.50 1.50 1.75 2.00 0.75 1.00 1.25 Protein concentration (mg/ml)
U. USU DU Protein concentration (mg/ml) Calculate the concentration of the unknown protein in mg/ml.
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