QUESTION 3 An unknown protein was determined using the following method: Biuret method Reagents Dissolve 4.0g sodium hyd

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Question 3 An Unknown Protein Was Determined Using The Following Method Biuret Method Reagents Dissolve 4 0g Sodium Hyd 1 (22.09 KiB) Viewed 71 times

Question 3 An Unknown Protein Was Determined Using The Following Method Biuret Method Reagents Dissolve 4 0g Sodium Hyd 2 (50.17 KiB) Viewed 71 times

Question 3 An Unknown Protein Was Determined Using The Following Method Biuret Method Reagents Dissolve 4 0g Sodium Hyd 3 (8.49 KiB) Viewed 71 times

Question 3 An Unknown Protein Was Determined Using The Following Method Biuret Method Reagents Dissolve 4 0g Sodium Hyd 4 (58.85 KiB) Viewed 71 times

QUESTION 3 An unknown protein was determined using the following method: Biuret method Reagents Dissolve 4.0g sodium hydroxide in about 400ml of distilled water. Add 4.5g sodium potassium tartrate. Mix to dissolve. Then add 1.5g CuSO4.5H20 followed by 4.5g potassium iodide Transfer the solution into a 500ml volumetric flask and make up to the mark with distilled water. Store in a tightly stoppered polyethylene bottle at room temperature (25-35°C). Stable for 6 months. Stock bovine immunoglobulin (Big) solution (10mg/ml). Dissolve 0.2g Big in 20mt distilled water, Working Big concentration range: 0.5 - 125 mg/ml
Method 1. Prepare the following test tubes and perform the method as indicated: Table 2: Preparation of test tubes for the biuret method Blank Tube 1 Tube 2 Tube 3 Tube 4 Distilled H20 1.0 ml Tube 5 Unknown 1.0 ml 0.25 mg/ml Big 0.5 mg/ml Big 0.75 mg/ml Big 1.0 ml 1.0 ml 1.0 ml 1.0 ml 1.0ml 1.0 mg/ml Big 1.25 mg/ml Big Unknown protein Biuret reagent 4.Oml 4.Oml 4.Oml 2. Mix thoroughly after each addition 3. Incubate at room temperature for 30 minutes. 4. Read absorbance at 540nm for all the tubes. 4.Oml 4.Oml 4.0ml 4.0ml
er the DOS Calculate the protein concentration of an unknown sample (in mg/ml) with an absorbancy value of 0.234 using the standard curve below.
1.5 1.4 13 1.2 1.1 10 0.9 0.8 Absorbancy 540nm 0.7 0.6 05 0.4 03 0.2 0.1 0.0 0.0 1.1 12 13 1.4 0.5 16 15 0.1 0.2 0.3 0.4 0.6 0.7 08 0.9 10 Big concentration (mg/ml)