You coculture your cells with conditioned media from different cells that make up the tumor microenvironment and look fo

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You coculture your cells with conditioned media from different
cells that make up the tumor microenvironment and look for changes
in their behavior. You notice that when you culture your cells in
conditioned media from fibroblasts (fibroblast CM), the cells in
culture undergo a change in their morphology.
How would you test further if these cells have undergone
transition to the mesenchymal state?