• During the PCR, the temperature change for certain times is designed in order to carry out the exponential amplification of the small DNA fragment by chain polymerization.
• Cycles are rendered in a basic three step process which is repeated 25-40 times:
• 1st step: separates the DNA double strand for a period of 15-40 seconds at a temperature of 95º C -97º C.
• 2nd step: designed primers react with the single stranded DNA thus sticking complementary to other bases in a period of 0.5-2min. at a temperature of 40º C- 65º C.
• 3rd step: dNTPs are placed between the primers synthesizing complementary sequence to template DNA strands for a period of 1-2 min. at a temperature of 72º C.
• During the PCR, the temperature change for certain times is designed in order to carry out the exponential amplificati
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• During the PCR, the temperature change for certain times is designed in order to carry out the exponential amplificati
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