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16.- Data showing whole blood (lysed with BD FACS TM lysing buffer) stained with a 13-color panel to identify various T-cell subsets (A). Tregs and memory cells (B). Acquisition and analysis were performed on BD-LSFRortessa TM (equipped with 5 laser). More information in

O CDB: T cells Effector Memory Central Memory Activated A CD45RO PE-Cy7 CD27 BV786 CD38 BV605 CD8 Naive Ellector 3 CCR7 BV 421 CD127 APC HLA-DR APC-H7 CDB FITC CO3+ T cells CD4+ T cells Effector Memory Central Memory Artvated CD3 PerCP-Cy5.5 CD4 BUV395 OUT CD45RO PE-cy? CD27 BV786 CD 98 V 605 Nave CR7 B1421 HLA-DR APCH7

Narker Fluorochrome Purpose Viability dye V500 Vlability CD3 PerCP-Cy5,5 T cell marker CD4 BUV395 T cell subsetting CD8 FITC T cell subsetting CD127 Alexa Fluor 647 Regulatory I Cell Marker HLA-DR APC-H7 Activation CD45RO PE-Cy7 Memory CD197 (COR) BV421 Naive/Memory CD38 BV605 Activation CD27 BV786 Memory CD25 PE CF594 Regulatory T Cell Market/Activation C0196 (CCRO) PE Thi? Tell Marker CXCR3 Alexa Fluor 700 Thcel Marker

Treg Memory Memory Activated B Treg CD127 APC CD45RO PE-Cy7 CD8 Name CD25 PE-CF594 HLA-DR APC-H7 CD8 FITC CD4- CD4 BUV395 CCR6 PE TH2 CXCR3 Alexa Fluoro 700

Write the phenotype of T regs memory activated T regs memory Th17 Th1