1. In DNA cloning, how are genetic markers related to transformation? 2. Why is it important to eliminate restriction sy

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1. In DNA cloning, how are genetic markers related to transformation? 2. Why is it important to eliminate restriction sy

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1. In DNA cloning, how are genetic markers related to transformation?
2. Why is it important to eliminate restriction systems in E. coli strains used in transformation
3. Abel runs a PCR on his samples using condition A, while Sibusiso and Tembisa used conditions B and C respectively for their PCR. Write about how the number of the cycles can affect their result (3 marks)
A. 10 cycles
B. 30 cyles
C. 50 cycles
4. Some water samples are brought to your laboratory and you have been asked to design a purification approach. Physical and chemical analysis of the sample indicates that the water has the following qualities: high concentration Ca and very dirty(brown). At which stage of the wastewater treatment are microbial contributions considered beneficial and significant? why?
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