This is question 8 and based on this question, answer the question below. Design a pair of CRISPR gRNAs to delete exon 5

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This is question 8 and based on this question, answer the
question below.
Design a pair of CRISPR gRNAs to
delete exon 51 out of gene DMD. Chromosome
coordinates are located within chrX:31,773,617-31,774,573 in
hg38. Hint: design gRNAs targeting the intron on each side
of the exon. Provide an explanation for partial
credit.
Design PCR primers to evaluate if the above question 8 (gRNA
pair) was successful. You can either design a single primer set to
detect two band sizes OR you can design two different primer sets
(one for unedited and one for edited). HINT: Consider the change in
size. Note that this isn’t qRT-PCR but a diagnostic PCR.