EXERCISE 14 Laboratory Report: Effects of Antibiotics Results Table 14.2 Antibiotic Susceptibility (Modified Kirby-Bauer

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Exercise 14 Laboratory Report Effects Of Antibiotics Results Table 14 2 Antibiotic Susceptibility Modified Kirby Bauer 1 (48.97 KiB) Viewed 103 times

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EXERCISE 14 Laboratory Report: Effects of Antibiotics Results Table 14.2 Antibiotic Susceptibility (Modified Kirby-Bauer Test) Antibiotic Pen (P-10) Zone S/UR (mm) Str (S-10) Zone S/L/R (mm) Tet (Te-30) Zone SA/R (mm) Cip (CIP-5) Zone S/I/R (mm) Sul (SKT) Zone SIUR (mm) Ery (5-15) Zone S/L/R (mm) S epidermidis Ecol M smegmatis A foecolis or P. oeruginoso Bocius subtills R-Resistant. I-Intermediate and S Susceptible Questions 1. What relationship did you find, if any, between the Gram-staining reaction of a microorganism and its susceptibility to an antibiotic? 2. Did you observe any small colonies growing in the zone of inhibition around the antibiotic dise? Explain how this might occur.

BIOL260 Lab 14: Antibiotics In this lab you will perform a modified Kirby-Bauer test to determine the antibiotic sensitivities of 4 different bacteria; E. coli, Staphylococcus aureus, Staphylococcus epidermidis, and Bacillus cereus. To set up the experiment the bacteria were inoculated to Mueller-Hinton agar using a sterile swab. The antibiotic discs were placed on the plates next using forceps, placing each disc evenly spaced from the others on the plate. All plates were inverted and placed in the incubator for 24 hours at 37°C. The images in this document reflect the plates after 24 hours of incubation at 37°C. The zones of inhibition for each disc have been measured with a ruler, measurements are in millimeters. What do you need to do for this lab? 1. Observe the plates and transfer the date from each plate to the Table 14.2. Note that this table has two different organisms listed, so please just make changes in the table to reflect the organisms in these images. You will need to change the table, adding the organisms Bacillus cereus and Staphylococcus aureus instead of M..smegmatis and P. aeruginosa. 2. The Table 14.2 has space to accommodate 6 antibiotics and the data provided has 8 drugs tested on each plate. Record all of the antibiotics EXCEPT Gentamicin and Polymixin B in the table. You will need to modify the table to reflect a few different antibiotics tested, ADD the drugs Chloramphenicol and Ampicillin to the chart and REMOVE Ciprofloxacin and Sulfonamides. Don't forget to record whether the organism is sensitive, intermediate, or resistant (S/I/R) in the Table 14.2 Use the antibiotic breakpoint table in this document to interpret the zones of inhibition 3. When interpreting the antibiotic Penicillin, remember that this is an antibiotic that only works against gram positive organisms. For E. coli please record the sensitivity as "N/A" since this drug would never be used to treat gram negative bacteria. Our lab book identifies one set of criteria as a breakpoint for Penicillin when testing with Staphylococcus and Streptococcus and a different set of values when interpreting Enterococcus. The values listed in the attached breakpoint table for Penicillin are for the gram positive bacteria listed in this lab (S. aureus, S epidermidis, and B. cereus). 4. Answer all the questions in the lab report, Q 1-5. Submit the lab to Canvas as a PDF. Data for the antibiotic lab is below, 4 plates and breakpoint table.

E 15 C 30 TE 30 GM 10 29mm 19mm 30mm 25mm 7mm B. cereus 8mm 21mm Omm S 10 AM 10 PB 300 P 10

PB 300 E 15 AM 10 GM 10 Omm 33mm 25mm Omm S. aureus 28mm Omm 1.8mm 14mm C 30 TE 30 S 10 P 10

S 10 TE 30 GM 10 PB 300 C 30 25mm 10mm 20mm 15mm 20mm S. epidermidis 30mm 25mm 30mm AM 10 E 15 P 10

AM 10 PB 300 P 10 E 15 Omm 14mm 10mm Omm E. coli 26mm 23mm 12mm 22mm S 10 GM 10 TE 30 C 30

Antimicrobial Agent Ampicillin (AM 10) when testing Staphylococci Chloramphenicol (C 30) Erythromycin (E 15) Gentamicin (GM 10) Penicillin G (P 10) when testing Enterococci Polymyxin B (PB 300) Streptomycin (S 10) Tetracycline (Te 30) Zone of Inhibition Diameters (mm) Susceptible Intermediate Resistant 217 14-16 313 229 None 328 218 13-17 312 223 14-22 513 215 13-14 312 29 528 215 None 314 212 9-11 38 215 12-14 311 219 15-18 314 None