Please answer all questions, i have been trying with these questions for 3 days. Please can someone answer them before t

[answerhappygod]

Please answer all questions, i have been trying with these
questions for 3 days. Please can someone answer them before
tomorrow!!!!!!! i have been struggling and really need
help.
1) The complementarity determining loops of an antibody are
responsible for the identification of the antigen using specific
epitope. Explain how the loops facilitate the recognition of
epitopes.
2) Briefly explain how the protein cleavage method for a
globular protein would differ from a membrane protein in
preparation for Liquid Chromatography-Mass Spectrometry (LC-MS)
experiments.
3) What general property would be expected of a liquid
chromatograhy (LC) column on which protein samples are being
separated for analysis by mass spectrometry? Briefly explain why
the column would have that property.
4) Briefly explain the importance of a positive control when
using chemical cleavage methods
5) Compare 2D polyacrylamide gel electrophoresis to LC-MS as a
proteomics technique to identify differences between patients with
diseases and controls without diseases.