- B A 104 Tyrosine Mrm Channel Standards L Tyr Phenylalanine Mrm Channel Standards L Phe 10 Cps Sda 2 5 D Phe 2 0 3 1 1 (53.19 KiB) Viewed 48 times
means?
Fig. 2.
Chiral amino acid analysis determines the chirality of amino
acid residues in a candidate
peptide. In this example of a chiral analysis on the extracted
Aplysia peptide GYFD, D-
tyrosine was detected in the tyrosine MRM channel (A) while only
L-phenylalanine was
detected in the phenylalanine MRM channel (B). This suggests
that the endogenous peptide
exists as GdYFD.
(B) (A) 104 Tyrosine MRM channel Standards L-Tyr Phenylalanine MRM channel Standards L-Phe 10 Cps sda 2.5 D-Phe 2.0 3 1.5 D-Tyr 2 1.0 1 0.5 0 0 Endogenous GYFD 6 D-Tyr L-Phe Endogenous GYFD 20 5 15 4 L-Tyr 3 10 5 2 5 1 0 0 16 17 21 22 13 اليا 14 17 18 18 19 20 Time (min) 15 16 Time (min)