4 Metformin is a first line treatment for type 2 diabetes. To detect the drug using liquid-chromatography with mass spec

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4 Metformin Is A First Line Treatment For Type 2 Diabetes To Detect The Drug Using Liquid Chromatography With Mass Spec 1 (46.29 KiB) Viewed 44 times

4 Metformin is a first line treatment for type 2 diabetes. To detect the drug using liquid-chromatography with mass spectrometry - Would you use positive or negative ion mode and give reason 2 Marks) b. Would you choose an acidic, basic or neutral mobile-phase and give reason (2 Marks) a. NH NH 'N N H NH2 Metformin (FM 129.167) C. d. Metformin elutes close to an impurity with a mass of 129,300. Calculate the resolving power of mass spectrometer required to detect both ions. [4 Marks) Pharmacokinetic profile of the drug was determined in two patients. Plasma samples were taken from patients from point of administration and thereafter for 12 h. The samples were subsequently analysed using tandem mass spectrometry. Standards of the drug in the concentration range 3.125 to 100 pg/mL were also analysed. Use the data provided to determine the concentration of drug in patient samples and use this to plot graphs showing how the concentration of drug in plasma varies with time. [12 Marks] Concentration of HPLC-speak area (arbitary metformin (c/mi) units) 3.125 6.25 153400 12.5 307685 25 614786 31.25 791020 50 1228990 100 2456780 76710 Time plasma sample taken th) 0 2 4 6 12 HPLC-peak area for HPLC-peak area for metformin (arbitrary units) metformin (arbitrary units) Patient 1 Patient 2 18434.7 24702.52 47910,6 64200.24 38085.3 51034.33 23347.4 31285.47 8609.4 11536.61