Procedures Day 1 Caution: Bleach is a strong oxidizer. Avoid skin contact and flush immediately with large amounts of wa

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Procedures Day 1 Caution: Bleach is a strong oxidizer. Avoid skin contact and flush immediately with large amounts of water if the solution gets on your skin. Note: Before filling your cuvette with a sample, do your best to dry the inside of the cuvette with a kimwipe so as not to change the concentration of the sample with residual liquid. Materials: 250 ml volumetric flask 50 ml beaker (6) 100 ml beaker 600 ml beaker Cuvette 25.00 mL volumetric pipet (shared) micropipettors 1. Appropriately dilute the stock solution of dye to 250.00 mL of a 10 x 106M solution. You will use this diluted solution for the kinetics experiment. 2. Obtain 10 ml of bleach solution in one 50 ml beaker and 10 mL of the 1.11 M NaCl solution in the other. 3. Open the "SpecCal" experiment file and calibrate the spectrometer procedure according to the following: a. If the message "no device connected" appears below the toolbar, go to the Experiment menu and select "Connect Interface" "Spectrometer" "Scan" b. The spectrometer must be calibrated before making any measurements. Go to the Experiment menu and select "Calibrate" "Spectrometer". The dialog box that appears will display a countdown timer to allow the spectrometer to warm up. Do not override this timer. When the countdown is finished, fill the cuvette half way with deionized water, place it in the spectrometer, and click "Finish Calibration". After a moment the "OK" button will become active. Click it to complete the calibration. 4. Obtain a UV/vis spectrum of the blue dye in the "SpecCal" file. Identify Amax and calculate & for the blue dye. Print a copy of your spectrum to turn in. Export the data onto your flash drive. 5. Open the "Kinetics" experiment file on the desktop. You will notice that there are three graph areas on this page: Adye vs. time (equation 11.4), In(Asya) vs. time (equations 11.5), and 1/Adve vs time (equation 11.6). 6. Pipet three aliquots of 25.00 ml 10 x 10 M FD&C blue dye into three separate 50 ml beakers. 7. Add 1.00 mL of undiluted bleach to one of the 25.00 mL aliquots of dye. Mix the reaction.

bleach react. 8. Condition the cuvette three times with the reaction solution and then place reaction mixture in the cuvette and hit "Collect" in LoggerPro. You can observe the excess dye/bleach mixture in the beaker to see what the spectrometer is measuring as the dye and 9. Collect data until the absorbance reaches approximately 0.20, click "Stop" 10. Empty and rinse the cuvette with deionized water. Look at the three graphs of data. Which appears linear? What does this indicate about the value of n, the order of the reaction with respect to (dye]? Click once on the linear graph to make it the active window and select the linear fit button. Record the equation of the link in your notebook. Look back at equation 11.4-11.6 and determine whether the slope of this line is equal to Kork. Record the value of K for this trial in your notebook 11. Repeat steps 7-10 for the remaining two aliquots of dye solution for a total of three identical trials for the undiluted bleach. 12. In order to determine the value of p, the order of the reaction with respect to [Naoci), two more trials need to be run at a different concentration of Naoci. Pipet 1.00 mL of bleach solution and 1.00 mL of the NaCl solution into a clean small beaker. Mix the solution thoroughly 13. Pipet three aliquots of 25.00 ml 10 x 10M FD&C blue dye into three separate 50 mL beakers 14. Add 1.00 mL of diluted bleach to one of the 25.00 mL aliquots of dye. Mix the reaction. 15. Condition the cuvette three times with the reaction solution and then place reaction mixture in the cuvette and hit "Collect" in LoggerPro. You can observe the excess dye/bleach mixture in the beaker to see what the spectrometer is measuring as the dye and bleach react 16. Collect data until the absorbance reaches approximately 0.20, click "Stop" 17. Repeat steps 14-16 for the remaining two aliquots of dye solution for a total of three identical trials for the undiluted bleach. The NaCl solution is used to maintain the same ionic strength. Ionic strength is a measure of the total amount of ionic charge in the solution. The ionic strength of the bleach solution would be greatly reduced if it were diluted with deionized water.

Procedures Day 2 1. Prepare 250.00 mL of 10 x 10 M FD&C blue dye using volumetric glassware. Pour into a beaker for storage. 2. Prepare your diluted bleach solution (concentration of your choosing) using the provided 1.11 M NaOCI and NaCl solutions in a small beaker. 3. Pipet three aliquots of 25.00 ml 10 x 10 M FD&C blue dye into three separate 50 mL beakers. 4. Open the "Kinetics" file on your desktop. 5. Calibrate the spectrometer with Dl water (refer to lab manual for a refresher on protocol). 6. Add 1.00 mL of diluted bleach solution to one of the 25.00 ml aliquots of dye. Mix the reaction. 7. Condition the cuvette three times with the reaction solution and then place reaction mixture in the cuvette and hit collect in LoggerPro. 8. Collect data until the absorbance reaches approximately 0.20 9. Linearly fit data to check that k' is appropriate based on your chosen dilution. 10. Once complete, export your data File > Export as... > CSV 11. Repeat experiment two more times for a total of three identical trials. a 12. Save or send yourself all CSV files for your final report.

Data for undiluted bleach solution: [Naoci) used in trial (after addition of dye) 25.0mL Which graph appears linear? Ist order Value of n Trial 1 Trial 2 Trial 3 Slope of best fit line from kinetics data 0.5251 -0.3 -0.5667 Value of ماماكن الحمله [57ما0.5

Data for 1:1 diluted bleach solution: [NaCl) used in trial (before addition of dye) [NaOCI] used in trial (after addition of dye) Trial 1 Trial 2 Trial 3 Slope of best fit line from kinetics data -0.30AS. 0.3aso -0.3400 Value of 0.30as 0.3250 0.3400 Value of p based on k' Data for diluted bleach solution (your chosen concentration): 1:4 [Naoci) used in trial (before addition of dye) [Naoci) used in trial (after addition of dye) Trial 1 Trial 2 Trial 3 Slope of best fit line from kinetics data 0.101001180 -0.145 Value of 0.1010 0.18 0.11045 Value of p based on Value of k Average value of k