cd Substrate Rate (ul NaOH min-1) mM Ab BC 0 1.0 1.0 4.0 8.0 12 8.0 12 18 16 20 28 32 28 35 64 32 38 a Titration with Na

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Cd Substrate Rate Ul Naoh Min 1 Mm Ab Bc 0 1 0 1 0 4 0 8 0 12 8 0 12 18 16 20 28 32 28 35 64 32 38 A Titration With Na 1 (122.48 KiB) Viewed 56 times

cd Substrate Rate (ul NaOH min-1) mM Ab BC 0 1.0 1.0 4.0 8.0 12 8.0 12 18 16 20 28 32 28 35 64 32 38 a Titration with NaOH b20 mM NaOH, 0.010 mg esterase 20 mM NaOH, 0.005 mg esterase 010 mM NaOH, 0.020 mg esterase 1.0 2.2 3.5 6.0 10.0 16.0 -Calculate the ratios of the reaction rates for substrate A and B (VA/VB) and for substrate B and C (vb/vc) in a mixture of 20 mM of A, 20 mM of B and 100 mM of C, when A, B and C were used as competitive substrates. The amount of esterase was 0.010 mg. -Calculate the concentrations of substrate A and B, in a mixture of A, B and C, that will give the following relationships between the rates: VA= VB= 5vc. The concentration of substrate C was 50 mM and the amount of esterase 0.010 mg. -Calculate the reaction rate for substrate A in a mixture containing 50 mM of substrate A and 50 mM of substrate B, when used as competitive substrates (esterase 0,020 mg).