- 25 Ashley Chromatography Bb Results 04 26 Due Di Mm 1 Ds Imm Yellow 1 A 20 2 4 24 Blue 3 4 24 2 8cm 28 Sborda A 7cm 1 (59.53 KiB) Viewed 18 times
- 25 Ashley Chromatography Bb Results 04 26 Due Di Mm 1 Ds Imm Yellow 1 A 20 2 4 24 Blue 3 4 24 2 8cm 28 Sborda A 7cm 2 (52.8 KiB) Viewed 18 times
- 25 Ashley Chromatography Bb Results 04 26 Due Di Mm 1 Ds Imm Yellow 1 A 20 2 4 24 Blue 3 4 24 2 8cm 28 Sborda A 7cm 3 (44.71 KiB) Viewed 18 times
- 25 Ashley Chromatography Bb Results 04 26 Due Di Mm 1 Ds Imm Yellow 1 A 20 2 4 24 Blue 3 4 24 2 8cm 28 Sborda A 7cm 4 (82.01 KiB) Viewed 18 times
& 50% Evey Dye getow di (mm) 10 ia ds (mm) 1 व RE 0.34 0.41 blue 1 12 10 30 0.40 arot red 1 15 pro aq 0.52 4 aucou green 1 10 30 0.83 2 20 0.67
Due ( 75% Shea dicmon) ds (mm) 24 mm ) OB yellow 1 0.125 2 3 ооо o blue 25 O reda 25 O o 25 a o green 2 O Te
Part 2: Using Your Solvent System The best way to test if two compounds are the same or not, is to mix them together and see if 2 spots resolve using a good chromatography system. One reason for that is that Rr values depend on many variables, so making a comparison in a single run is always the best. You'll do that now to see if the green is composed of the same yellow and blue dye that are used for those colors. To make this test better you will make initial spots that are as weak as you can meaningfully see, but not so weak you won't be able to see the spots after you elute with the solvent mixture you picked. 1) In the corner of two small containers tilted so drops will stay where you put them, put two drops of green food color. 2) To one add a single drop of blue coloring, and to the other add a single drop of yellow. Mix them with a spotting tube. 3) On a single piece of chromatography (filter) paper, put two light spots of each of the mixtures and two spots of just green coloring. Try for very light spots, but ones where you'll be able to see the eluted spots easily. One of each pair of spot will likely be darker than the other one, which is okay. 4) Elute thr: chromatography paper as before using your optimized solvent system. Lab Report Follow the standard format for your lab report. Include a table of Rr values in all four solvents that is easy for a naive reader to understand. Explain your choice of solvent system for the follow-up experiment where you looked closer at the green food coloring, as well as the results you got. Is the green food color made up of the same blue and yellow that those food colors contain? What can you be certain of?