1. Two components elute closely at 4.80 and 5.00 minutes on a GC column that has an air peak of 0.3 minute. A chromatogr

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1 Two Components Elute Closely At 4 80 And 5 00 Minutes On A Gc Column That Has An Air Peak Of 0 3 Minute A Chromatogr 1 (228.15 KiB) Viewed 33 times

1. Two components elute closely at 4.80 and 5.00 minutes on a GC column that has an air peak of 0.3 minute. A chromatographic resolution of 1.00 was achieved with this column of 15 meters. How long should the column be in order to obtain a resolution of 1.50 to avoid difficulty in peak integration? What GC parameters need to be adjusted to maintain the same H value and give the numerical value of H? Assume that all the column parameters except the length are the same. 2. An open tubular column is 30.1 m long and has an inner diameter of 0.530 mm. It is coated on the inside wall with a layer of stationary phase that is 3.1 µm thick. Unretained solute passes through in 2.16 min, whereas a particular solute has a retention time of 17.32 min. (a) Find the linear and volume flow rates. (b) Find the retention factor or capacity factor for the solute and the fraction of time spent in the stationary phase. (c) Find the partition coefficient for this solute.