8) (6-points) If you have a mixture of aspartic acid, valine and histidine, and you want to separate them by electrophor

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8 6 Points If You Have A Mixture Of Aspartic Acid Valine And Histidine And You Want To Separate Them By Electrophor 1 (228.07 KiB) Viewed 34 times

8) (6-points) If you have a mixture of aspartic acid, valine and histidine, and you want to separate them by electrophoresis in such a way that they travel in opposite directions in the electrophoresis apparatus, what is the optimal pH range for your buffer? Describe the direction of each amino acid on the electrophoresis plate. Use the Isoelectric Point (pl) on the following table to answer this question. Amino Acid Isoelectric Point (pl) Isoelectric Point Amino Acid (pl) glycine 6.0 threonine 5.6 alanine 6.0 tyrosine 5.7 valine 6.0 cysteine 6.1 leucine 6.0 asparagine 5.4 isoleucine 6.0 glutamine 5.7 aspartic phenylalanine 5.5 2.8 acid glutamic methionine 5.7 3.2 acid proline 6.3 histidine 7.6 tryptophan 5.9 lysine 9.7 serine 5.7 10.8