7. Chromatography theory a) A key equation in chromatography theory is the resolution equation, which looks like this N

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7 Chromatography Theory A A Key Equation In Chromatography Theory Is The Resolution Equation Which Looks Like This N 1 (34.22 KiB) Viewed 21 times

7. Chromatography theory a) A key equation in chromatography theory is the resolution equation, which looks like this N R What value of R is needed in order to achieve base-line resolution of two adjacent peaks in a chromatogram? b) Take a look at the chromatograms below, which show the resolution for two peaks varying from 0.50 to 1.50 (the figure below is from your textbook). Explain briefly how the resolution values are obtained in each chromatograph. That is, what is the other formula for calculating resolution that I gave you in your chromatography theory handout? 20 Peson -0.50 Reuton -0.76 Resolution 1.50 N Baseine resolution Time c) In the resolution equation above, explain what a is, and what the minimum value of alpha must be in order to achieve optimal resolution between two adjacent peaks. d) In the resolution equation above, explain what N is, what the most desirable values of Nare, and what chromatographic parameters have the greatest affect on N. 7 más 11 Reso -100 -bg mas