Bacteria Identification: Escherichia coli Standards for antimicrobial disc diffusion susceptibility tests tror ctarryoce
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Standards for antimicrobial disc diffusion susceptibility tests
tror ctarryoceco Vor erieromono Rey stactionoco
Example of Mueller-Hinton Agar plate with bacterial lawn-fype growth incubated with 7 different antibiotics of known concentration (white discs) and a control. Measuring the diameter of the Zone of Inhibition of each antibiotic and its effect upon the bacterial culture allows for the determination of Minimum Inhibitory Concentrations of antibiotics necessary to inhibit growth of specific bacterial cultures (Image from http://www.asm.org/visuallibrary). The Kirby-Bauer method can also be used to test the efficacy of newly developed antibiotics against known bacterial cultures. Furthermore, as bacterial strains develop resistance to certain antibiotics, physicians must change medications and use other antibiotics according to their ability to also kill a bacterial strain causing an infection. In medicine, it is important to use the most effective antibiotic, or the one with the lowest MIC to still create a desired ZOI in bacterial growth. By this point in the semester, students should already have a tentative identification of their bacterial isolate. With this knowledge in mind, ask yourself whether the antibiotic sensitivity results seen in this Kirby Bauer experiment make sense with your tentative bacterial identification. For example, if you see the sensitivity of your bacterial isolate toward a specific antibiotic, does this sensitivity coincide with your research about your tentative bacterial species (or genus) identification?
ACCEPTABLE IDENTIFICATION \begin{tabular}{|l|c|c|c|c|c|c|c|} \hline Significant taxa & % ID & T & \multicolumn{3}{|l|}{ Tests against } \\ \hline Bacillus megaterium & 84.6 & 0.78 & DXYL 76\% & XLT & 11% & & \\ \hline \end{tabular} \begin{tabular}{|l|c|c|c|c|c|c|c|} \hline Next taxon & %10 & T & \multicolumn{2}{|c|}{ Tests against } \\ \hline Paenibacillus glueanolyticus & 8.7 & 0.58 & DXYL 99% & INO & 14\% & XLT & 5% \\ \hline \end{tabular}