Answer Happy

1. Review the example in Chapter 15 , slides 15 to understand the concept of reaction coupling to make an unfavorable process (phosphorylation of glucose) thermodynamically favorable. 2. Apply this principle to understand how reaction coupling enables ATP synthesis by substratelevel phosphorylation: In glycolysis, coupled reactions produce net ATP from the oxidation of a molecule of glucose. One of the ATP-producing steps of glycolysis is the substrate-level phosphorylation reaction catalyzed by pyruvate kinase: Phosphenolpyruvafe Pyruvate which we will abbreviate as: PEP + ADP → pyruvate + ATP
If the hydrolysis of PEP (that is, PEP +H2​O→ pyruvate +Pi​ ) has a ΔG0∘=−61.9 kJ/mol, and if the hydrolysis of ATP (that is, ATP +H2​O→ ADP +Pi ​ ) has a ΔG∘=−30.5 kJ/mol, then what is the ΔG∘ value for the coupled reaction PEP + ADP → pyruvate + ATP?