Answer Happy

a) Define enzyme inhibitor. Briefly explain the reasons behind the differences between uncompetitive and non-competitive enzyme inhibition by sketching the respective Lineweaver-Burk double reciprocal plot. Can raising the substrate concentration decrease both types of inhibition? Explain your answer. (10 marks) b) The following is a synthesized tripeptide. i. Predict and sketch the final structure of the tripeptide when immersed in a pH6 buffer. ii. Predict and sketch the final structure of the tripeptide when immersed in a pH9 buffer. (4 marks) c) State and define the two (2) classes of amino acids based on nutrition. Provide one (1) example for each class. (6 marks) d) Briefly describe the principles of gel filtration chromatography for the treatment of protein.