Separation principles in capillary electrophoresis involved the movement of ions in a silica capillary column because of
Posted: Fri Jul 15, 2022 4:45 pm
Separation principles in capillary electrophoresis involved themovement of ions in asilica capillary column because of electrophoretic flow andelectroosmotic flow (EOF).i) Differentiate ions movement between electrophoretic flow (EF)and electroosmoticflow (EOF). (6 marks) ii) Explain two approaches that can be used to reduce theEOF.(4 marks)b) Discuss the best electrophoresis technique/mode than can beapplied for the followingseparations. In each case,
(i) name the recommended technique, (ii) give specificexperimental conditions required and (iii) explain the expectedorder of migration of thespecies involved. Give reasons in each case.i) 35Cl- and 79Br- (5 marks)
ii)Protein mixture (molecular weight, Mw10000 to 20000)
(i) name the recommended technique, (ii) give specificexperimental conditions required and (iii) explain the expectedorder of migration of thespecies involved. Give reasons in each case.i) 35Cl- and 79Br- (5 marks)
ii)Protein mixture (molecular weight, Mw10000 to 20000)