we nave data for Nad not for albumin Stodk sol conC=2.5914 For this we will use molarity equation * Stock onoles = Patie
Posted: Fri Jul 15, 2022 8:31 am
- We Nave Data For Nad Not For Albumin Stodk Sol Conc 2 5914 For This We Will Use Molarity Equation Stock Onoles Patie 1 (131.22 KiB) Viewed 46 times
- We Nave Data For Nad Not For Albumin Stodk Sol Conc 2 5914 For This We Will Use Molarity Equation Stock Onoles Patie 2 (154.53 KiB) Viewed 46 times
Part I: Calibration Curve Data Entry Table 2. Standard curve absorbance data Volume of standard solution added to each cuvette Vnlume of RC.G solution added to each couvette 0.50 '.1pts) Enter the slope of the trendline in your plot. 1pts) Enter the y-intercept of the trendline in your plot. Using the concentration and absorbance measurements in Table 5, produce a calibration plot. Uploac (.jpeg, .png, or. pdf) of your calibration plot. Upload the plot here. Do not upload a .xlsx (Excel) or doc file. (4.5pts) Creating Standards Using the stock solution given, enter the volumes used to prepare the six standard solutions of albumin in NaCl solution to represent 40-fold dilutions of typical "patient" concentrations from 20 to 70 g/L. For each standard the target "patient" concentration is given, you need to record the actual concentration of the standard, the volume of the stock solution used to make the standard, and the volume of NaCl solution used to make the standard. We are assuming you are making 10 mL of each standard solution. NOTE: If you ever need to write a number using scientific notation at any time in this report, use "E" notation (i.e. 1.5E-3) Stock solution concentration: 2.5 g/L Part III. Spectrophotometric Analysis of Patient Samples Data Entry Ptock solh conc =2.5glL. Volume of patient sample added to the cuvette: 0.50 mL For this we will exe retoritg equetion Volume of BCG added to the cuvette: 1.00 mL St.13 V2=2.5200×10≤120 onl Using the calibration curve from Part, calculate the concentration for the selected patients shown in the tabl Sidu. V4=2.5500×10=2Com ? Note: The patient concentrations in shown in this part may not correspond to Part III. Volume of patient sample added to the cuvette: 0.50 mL S1d5. V5=2.560c×10=24cml Volume of BCG added to the cuvette: 1.00 mL shde YE=2.570co×10=290ml Tahle 7 Pationt samnles ahsorhanres and canneentration