When designing primers that will have an added restriction enzyme site, you should a. add extra bases 3' (upstream) to t
Posted: Tue Jul 12, 2022 1:27 pm
When designing primers that will have an added restrictionenzyme site, you should
a.
add extra bases 3' (upstream) to the restriction enzymesite.
b.
ensure that the restriction site has an affinity tag.
c.
ensure that the restriction site is complimentary to the targettemplate.
d.
ensure that the cytosines on the restriction sites aremethylated.
e.
add extra bases 5' (upstream) to the restriction enzymesite.
a.
add extra bases 3' (upstream) to the restriction enzymesite.
b.
ensure that the restriction site has an affinity tag.
c.
ensure that the restriction site is complimentary to the targettemplate.
d.
ensure that the cytosines on the restriction sites aremethylated.
e.
add extra bases 5' (upstream) to the restriction enzymesite.