Answer Happy

You have been performing a PCR reaction but your results aren'tthe greatest. Your Supervisor has told you that you should increasethe concentration of Magnesium. What affect will this have on thereaction?
a.
The annealing temperature will decrease.
b.
The annealing temperature will not be affected but the enzymeactivity will be affected.
c.
The Annealing temperature will increase.
d.
The denaturation temparture will have to be decreased in the PCRprotocol.
e.
The denaturation temparture will have to be increased in the PCRprotocol.