Answer Happy

OBSERVATIONS Chemotherapeutic Agent Ampicillin (AM10) Chloramphenicol (C30) Ciprofloxin (CIP5) Erythromycin (E15) Cefoxitin. (FOX30) Gentamicin (GM10) Novbiocin (NB30) Penicillin (P10) Streptomycin (510) Trimethoprim sulfamethoxazole (TMP-SMZ) Tetracycline (TE30) Vancomycin (VA30) E. coli Zone Size Susceptibility 45. thim 25 30 S 9 mm 20 mm 7 m 20 mm 20 Pem 24 man S 1 mm R S T R 5 Gram-negative S S R P. aeruginosa Zone Size Susceptibility mm 28 25 mm 22 num 21 nim 7 = 7 7 7 R S 12. S R R a d 12. R Zone Size 425 mm 25 D 28 24 32 S. aureus 40 mm S 28 mm 18 Them 26 mm 21 15 mm Susceptibility S S 5 S 5 3 S S Gram-positive S S S E. faecalis Zone Size Susceptibility 29. mm 15 22 mm 22 mm. mm 17 mm mm 7 mm 7 mm 12 mm. 9 m S H I H A H L R R I R 18 5 73 NOTE: In actual clinical testing of bacterial isolates from patients, inoculum turbidity is precisely stan- dardized to 10 cfu/ml and Mueller-Hinton agar is used. Using these standardized conditions allows valid comparison to the cut-off points listed. Experiment 24 117

2. Which is most effective or appears to be a broad-spectrum antibiotic 3. Which antibiotic is less effective against Gram positive bacteria 4. Which antibiotic is less effective against Gram- Negative bacteria