Answer Happy

Serial Dilution Diagram 1. Using the diagram below, answer the following questions. From each tube, 10 ml was removed and mixed with nutrient agar and a pour plate was made. The results were: Original complete growth, no individual colonies; A = complete growth, no individual colonies; B = 367 colonies; C= 64 colonies; D = 7 colonies Original A= B = C= What are the final dilutions of each tube as compared to the original sample? What is the concentration of bacteria/ml? D= 1.0 ml 10 ml A 1.0 ml 9 ml Bacterial concentration = B 1.0 ml 9 ml C 1.0 ml 9 ml D 9 ml

Plating 2. Give the dilution of each of the tubes and the dilution of each of the plates. (2.5 pts) 1mL Original inoculum 0.1 mL F 9 mL 1 mL G 9 ml B 1 mL 9 mL C H 1 mL 9mL O 1 mL 9mL E

A = B= C= D= E = F= G= H= |= J= F G H