C. Questions 1. What is the maximum OD that is within the linear portion of the curve? 2. What is the corrected or true
Posted: Tue Jul 12, 2022 1:22 pm
C. Questions 1. What is the maximum OD that is within the linear portion of the curve? 2. What is the corrected or true OD of the undiluted culture? (Hint: If the OD for the 1:2 dilution, but not the 1:1 diluti is within the linear portion of the curve, then the OD of the 1:1 dilution should not be considered correct. The correct or OD of the undiluted culture in this example could be estimated by multiplying the OD of the 1:2 dilution by 2. 3. What is the correlation between corrected OD and cell number for your culture? 4. Why is it necessary to perform a plate count in conjunction with the turbidimetry procedure? 5. If your medium were pale blue instead of amber-colored, as is the case of nutrient broth, would you set the wavelengt control knob higher or lower than 686 nanometers?