Hey, can you check over my answers? Can you correct the ones that are wrong?
Posted: Tue Jul 12, 2022 1:21 pm
Hey, can you check over my answers? Can you correct the onesthat are wrong?
Question 25 cuts DNA molecules at specific locations A. restriction enzymes OB. gene cloning OC. DNA ligase OD. gel electrophoresis O E. reverse transcriptase
How does a genomic library differ from a cDNA library? OA. A genomic library contains both noncoding sequences and coding sequences, whereas a cDNA library contains only coding sequences. OB. A genomic library is identical regardless of the cell type used to make it, whereas the content of a cDNA library depends on the cell type used in its construction. OC. A genomic library can be made using a restriction enzyme and DNA ligase only, whereas a cDNA library requires both of these as well as reverse transcriptase and DNA polymerase. OD. B and C are correct. OE. A, B and C are correct.
Question 27 The polymerase chain reaction is important because it allows us to: OA. insert eukaryotic genes into prokaryotic plasmids. OB. incorporate genes into viruses. OC. make DNA from RNA transcripts. D. make many copies of a targeted segment of DNA. E. insert regulatory sequences into eukaryotic genes. 0.
Question 28 PCR method is used for the following purposes: A. Gene cloning OB. diagnosis of genetic disease C. forensic laboratory OD. All of the above
Question 29 Each cycle of PCR consists of: OA. DNA denaturation OB. DNA annealing OC. DNA synthesis OD. All of the above
Question 30 Restriction fragments of DNA are typically separated from one another by which process? OA. filtering B. centrifugation OC. gel electrophoresis 0.5 points OD. PCR
Question 31 Probes are short, single-stranded DNA segments that are used to identify DNA fragments with a particular sequence. In order to identify a specific restriction fragment using a probe, what must be done? OA. The fragments must be separated by electrophoresis. B. The fragments must be treated with heat or chemicals to separate the strands of the double helix. OC. The probe must be hybridized with the fragment. 0.5 points Save Answer D. All of the above.
Question 32 DNA fragments from a gel are transferred to a nitrocellulose paper during the procedure called Southern blotting. The purpose of transferring the DNA from a gel to a nitrocellulose paper is to: A. permanently attach the DNA fragments to a substrate. B. separate the two complementary DNA strands. OC. transfer only the DNA that is of interest. D. 0.5 points Save Answer prepare the DNA for digestion with restriction enzymes.
- Hey Can You Check Over My Answers Can You Correct The Ones That Are Wrong 1 (84.14 KiB) Viewed 44 times
How does a genomic library differ from a cDNA library? OA. A genomic library contains both noncoding sequences and coding sequences, whereas a cDNA library contains only coding sequences. OB. A genomic library is identical regardless of the cell type used to make it, whereas the content of a cDNA library depends on the cell type used in its construction. OC. A genomic library can be made using a restriction enzyme and DNA ligase only, whereas a cDNA library requires both of these as well as reverse transcriptase and DNA polymerase. OD. B and C are correct. OE. A, B and C are correct.
Question 27 The polymerase chain reaction is important because it allows us to: OA. insert eukaryotic genes into prokaryotic plasmids. OB. incorporate genes into viruses. OC. make DNA from RNA transcripts. D. make many copies of a targeted segment of DNA. E. insert regulatory sequences into eukaryotic genes. 0.
Question 28 PCR method is used for the following purposes: A. Gene cloning OB. diagnosis of genetic disease C. forensic laboratory OD. All of the above
Question 29 Each cycle of PCR consists of: OA. DNA denaturation OB. DNA annealing OC. DNA synthesis OD. All of the above
Question 30 Restriction fragments of DNA are typically separated from one another by which process? OA. filtering B. centrifugation OC. gel electrophoresis 0.5 points OD. PCR
Question 31 Probes are short, single-stranded DNA segments that are used to identify DNA fragments with a particular sequence. In order to identify a specific restriction fragment using a probe, what must be done? OA. The fragments must be separated by electrophoresis. B. The fragments must be treated with heat or chemicals to separate the strands of the double helix. OC. The probe must be hybridized with the fragment. 0.5 points Save Answer D. All of the above.
Question 32 DNA fragments from a gel are transferred to a nitrocellulose paper during the procedure called Southern blotting. The purpose of transferring the DNA from a gel to a nitrocellulose paper is to: A. permanently attach the DNA fragments to a substrate. B. separate the two complementary DNA strands. OC. transfer only the DNA that is of interest. D. 0.5 points Save Answer prepare the DNA for digestion with restriction enzymes.