Question: Some of the earliest experiments on the cytoskeleton involved squeezing the axoplasm from squid giant axons on
Posted: Tue Jul 12, 2022 1:18 pm
Question:
Some of the earliest experiments on the cytoskeleton involvedsqueezing the axoplasm from squidgiant axons onto a slide. Then, the scientists would observe themovements of cytoskeletal filaments onthe slide. Figure Q2-10 shows a drawing of one of theseexperiments: a substance purified from squidaxoplasm had been immobilized on a glass slide, and a microtubuleis shown moving rightward on theslide, in the presence of ATP.Flgure Q2-10A. What substances might have been purifled to allow movement ofthe micretubule? In 1-2sentences, explain your reasoning.Key polnts:(2) (2 polnts) Kinesins or dynein were most likely purified fromthe axoplasm to allew movement ofthe microtubule. (need both for full credit)(2) (2 points) These are micretubule metors. If they were stuck tothe side, they would move themicrotubule since they can't move the slide.B. In 1-3 sentences, indicate what you would need to know about themicrotubule to distinguishbetween the possible substances that you listed in A? Explain yourreasoning.Key points;(1) (1 point) You would need to know which end of the microtubuleis the plus end (or minus end).(2) (3 points) Normally, kinesin moves cargo toward the plus end,while dynein moves cargo towardthe minus end. Therefore, knowing which end is plus versus minuswould tell you which directionthe motor wants to move. This would tell you whether the purifiedprotein is kinesin or dynein.
My answer:
a) Microtubules are hollow, fibrous shafts whose essentialfeature is to assist help and give form to the cell. they alsoserve a transportation characteristic, as they may be the routesupon which organelles circulate via the cellular. they may be mostoften determined in all eukaryotic cells and, collectively with themicrofilaments and intermediate filaments, form thecytoskeleton.
b) Microtubules have many greater jobs than simply givingassist to the cell. The microtubules additionally play a very vitalrole at some point of cell department. Their number one mobiledivision feature is to connect with the chromosomes, assist theones chromosomes whole their first cut up, after which pass thebrand new chromosomes to their places in the new daughter cells.Microtubules are key players in cellular self-organization, actingas structural scaffolds, cellular highways, force generators andsignalling platforms. Microtubules are polar filaments that undergodynamic instability, i.e. transition between phases of growth andshrinkage. This allows microtubules to explore the inner space ofthe cell, generate pushing and pulling forces and remodelthemselves into arrays with different geometry and function such asthe mitotic spindle. To do this, eukaryotic cells employ an arsenalof regulatory proteins to control microtubule dynamics spatiallyand temporally. Plants and microorganisms have developed secondarymetabolites that perturb microtubule dynamics, many of which are inactive use as cancer chemotherapeutics and anti-inflammatory drugs.Here, summarizing the methods used to visualize microtubules and tomeasure the parameters of dynamic instability to study bothmicrotubule regulatory proteins and the action of small moleculesinterfering with microtubule assembly and/or disassembly.
Please let me know which key points I missed for both part A andB, and how I might improve upon these for the future
Some of the earliest experiments on the cytoskeleton involvedsqueezing the axoplasm from squidgiant axons onto a slide. Then, the scientists would observe themovements of cytoskeletal filaments onthe slide. Figure Q2-10 shows a drawing of one of theseexperiments: a substance purified from squidaxoplasm had been immobilized on a glass slide, and a microtubuleis shown moving rightward on theslide, in the presence of ATP.Flgure Q2-10A. What substances might have been purifled to allow movement ofthe micretubule? In 1-2sentences, explain your reasoning.Key polnts:(2) (2 polnts) Kinesins or dynein were most likely purified fromthe axoplasm to allew movement ofthe microtubule. (need both for full credit)(2) (2 points) These are micretubule metors. If they were stuck tothe side, they would move themicrotubule since they can't move the slide.B. In 1-3 sentences, indicate what you would need to know about themicrotubule to distinguishbetween the possible substances that you listed in A? Explain yourreasoning.Key points;(1) (1 point) You would need to know which end of the microtubuleis the plus end (or minus end).(2) (3 points) Normally, kinesin moves cargo toward the plus end,while dynein moves cargo towardthe minus end. Therefore, knowing which end is plus versus minuswould tell you which directionthe motor wants to move. This would tell you whether the purifiedprotein is kinesin or dynein.
My answer:
a) Microtubules are hollow, fibrous shafts whose essentialfeature is to assist help and give form to the cell. they alsoserve a transportation characteristic, as they may be the routesupon which organelles circulate via the cellular. they may be mostoften determined in all eukaryotic cells and, collectively with themicrofilaments and intermediate filaments, form thecytoskeleton.
b) Microtubules have many greater jobs than simply givingassist to the cell. The microtubules additionally play a very vitalrole at some point of cell department. Their number one mobiledivision feature is to connect with the chromosomes, assist theones chromosomes whole their first cut up, after which pass thebrand new chromosomes to their places in the new daughter cells.Microtubules are key players in cellular self-organization, actingas structural scaffolds, cellular highways, force generators andsignalling platforms. Microtubules are polar filaments that undergodynamic instability, i.e. transition between phases of growth andshrinkage. This allows microtubules to explore the inner space ofthe cell, generate pushing and pulling forces and remodelthemselves into arrays with different geometry and function such asthe mitotic spindle. To do this, eukaryotic cells employ an arsenalof regulatory proteins to control microtubule dynamics spatiallyand temporally. Plants and microorganisms have developed secondarymetabolites that perturb microtubule dynamics, many of which are inactive use as cancer chemotherapeutics and anti-inflammatory drugs.Here, summarizing the methods used to visualize microtubules and tomeasure the parameters of dynamic instability to study bothmicrotubule regulatory proteins and the action of small moleculesinterfering with microtubule assembly and/or disassembly.
Please let me know which key points I missed for both part A andB, and how I might improve upon these for the future