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Materials 1 Large potato tuber Cork borer Razor blade Metric ruler Distilled water Sucrose solutions: 0.1, 0.2, 0.3, 0.4, 0.5, 0.6 M Procedure In this procedure, you will use a cork borer and a sharp razor blade. Use extreme caution and technique to avoid personal injury to your hands and fingers! 1. Label your 7 disposable cups or beakers as 0, 0.1, 0.2, 0.3, 0.4, 0.5, and 0.6. 2. Place 100 ml of distilled water in the O-marked cup, and 100 ml of each of the sucrose solutions into their labeled cups. 3. Place the potato tuber with its narrow side on the table. A position that will allow you to cut 7 potato cylindrical pieces > 5 cm in length with the cork borer. 4. Cut out seven potato cylinders with the cork borer by pushing and twisting while the potato tuber is on the table (Not in your hand!) and place the separate pieces in the petri dish. 5. Use the metric ruler and the razor blade to trim all cylinders to 5 cm removing the potato skin on either side of the cylinder. 6. Pat dry one of the cylinders to remove any excess water of the side. 7. Weigh the potato cylinder and enter the weight in table 5, below. 8. Cut each cylinder into two long halves using your razor blade and place into the water beaker.and Record the time 9. Repeat steps 6 to 8, and place each two halves of the same cylinder solutions starting with the 0.1 m and ending with 0.6 M. 10. Incubate all pieces in their solutions for 1.5-2.0 hrs, swirling the content every 15 minutes. 11. Record the time at the end of the incubation period. 7 disposable cups or beakers Weighing scale Petri dish Forceps Aluminum foil 12. Calculate the approximate incubation time. 13. Remove the pieces starting with the one in the distilled water, using the forceps, pat dry and weigh. Enter the final weight of the two in table 5, below. +++ Change in weight (g) % change in weight 14. Repeat step 13 for the remaining pieces following their order of incubation. 15. Enter all final weights in table 5. 16. Calculate the change in weight of each two pieces by subtracting the initial weight from the final weight and enter in the table. Remember to indicate the positive increase as "+" and the decrease as "-". 17. Calculate the % change in weight for each sample and enter in table 5, as % change in weight = (final weight-initial weight) X 100 initial weight Table 5. Results/Data of estimation of potato cell osmolarity by change in weight Sucrose Molarity (M) 0.3 5.52 5.36 0 Initial weight (g) 5. 65 Final weight (g) G. 21 0.56 9.91% 5.56% 0.1 0.2 5.58 5.50 5.89 5.64 0.31 0.14 one sucrose 0.4 0.5 0.6 5.40 5.38 5.3) 5.93 4.41 4.16 0.50 -0.97 -0.16 -1.15 2.45% -2.90% 9.26% -18.03% -21.66
Estimation of Osmolarity of Potato Cells Scientific Report State the question you are attempting to answer in this lab exercise? What is the osmolarity of potato cell? State your own hypothesis. U State your own prediction. 14 th Sucrose 1 Results Present your data clearly in table form in the provided space. The table must be clearly understood and properly labeled (Table #, title, columns with titles and units etc....). of % change in weight Present your data clearly in a figure using the appropriate graphical plot. This must be clearly understood and properly labeled (Figure #, title, legend, units etc).
percent change weight Describe the results in your own words referring to the table and the figure, citing both in the text the figure and table numbers for easy reference. Make sure to put observations ONLY, including the point of intersection of the best fit line with the X-axis.