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6.DISCUSSION: Write in paragraphs Pour Plate Method A- B- State the total colony count (number of colonies/ plate) using both ColonyDoc-it count and '10 Square' Calculation method. Discuss two reasons why the total colony counts are different. Explain which method is more accurate and give one reason why. Explain why the results of pour plate count are expressed as CFU/mL and not bacteria / mL. Explain one problem were encountered with the pour plate method and its effect on your final results Turbidity Method 67 For the turbidimetric method, discuss whether or not the data yielded a straight line or was it curved. Use information like the r2 value to justify your answer. If you had used the '10 square' calculated method for the determination of x-axis) of the plot instead of the 'ColonyDoc-it' counter values, how would the linear regression equation changed in terms of slope value. (Calculate the slope to prove your answer) Explain how using the 10 square calculated result have would affect your final result for the determination of the CFU/mL in the unknown E.coli culture. Discuss one error encountered with the turbidimetric method and it's effect on your final result? 7.Post Lab Questions: 71/105 1. Explain why pour plate method is known as minimal counts. 2. Explain why turbidity measurements are known as total counts. 3. Explain why optical density is plotted instead of % Transmittance on the y-axis. 4. Explain why the 100% Transmittance was set to the broth and not with water. 5. Explain what you would have likely observed if a 1ml sample from Blank A would have been plated ::