1. A diluted sample with a concentration of 10 ug/ul with a dilution factor of 5 is given. Calculate the original concen
Posted: Mon Jul 11, 2022 2:18 pm
1. A diluted sample with a concentration of 10 ug/ul with adilution factor of 5 is given. Calculate the original concentrationfrom the stock solution.
2.The performing of a PCR to amplify two genes in a unknownsample, as David decides to run a agarose gel for furtherconfirmation of the presence of the amplicons with is expected sizeof 2100 bp and 2600 bp. Decide on the % of the agarose (in any fromthe 0.5-1.5%) and the determine how much of the agarose does Davidneed to weigh for the gel of 75 ml.
Percent Agarose gel (w/v) DNA Size resolution (kb=1000)
0.5% 1 kb to 30 kb
0.7% 800 bp to 12 kb
1.0% 500 bp to 10 kb
1.2% 400 bp to 7kb
1.5% 200 bp to 3 kb
2.0% 50 bp to 2 kb
2.The performing of a PCR to amplify two genes in a unknownsample, as David decides to run a agarose gel for furtherconfirmation of the presence of the amplicons with is expected sizeof 2100 bp and 2600 bp. Decide on the % of the agarose (in any fromthe 0.5-1.5%) and the determine how much of the agarose does Davidneed to weigh for the gel of 75 ml.
Percent Agarose gel (w/v) DNA Size resolution (kb=1000)
0.5% 1 kb to 30 kb
0.7% 800 bp to 12 kb
1.0% 500 bp to 10 kb
1.2% 400 bp to 7kb
1.5% 200 bp to 3 kb
2.0% 50 bp to 2 kb