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7. From "Wikipedia": Zidovudine (ZDV), also known as azidothymidine (AZT), is an antiretroviral medication used to prevent and treat HIV/AIDS. It is generally recommended for use with other antiretroviral. It may be used to prevent mother to child spread during birth or after a needlestick injury or other potential exposure. Here is the structure of AZT: HO- HN N₂ N CH3 How does this work to treat HIV?
8. Why is it a good idea to design primers for PCR that end with at least two G's or C's at the 3' end? 3, Primer, 3' '5' 3' primer 9. The following two radioactively-labeled nucleotides are sold by GE Amersham: Adenosine 5'-[-³2P]triphosphate, triethylammonium salt Adenosine 5'-[y-32P]triphosphate, triethylammonium salt Which would you use to measure the rate of DNA replication? Explain how this would work?
2. In eukaryotic cells, why does it take longer to carry out translation once transcription is completed? 3. Where are codons located? 4. What enzyme is needed for transcription? 5. During translation, what amino acid is always added first? 6. Is tRNA single-stranded or double-stranded? 7. What two important sites are found on tRNA? 8. Why can ribosomes be found in two different locations in the cell? Explain how their job differs depending upon their location?