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ige 1: 2- 25 5 1- GFP is approximately__kD. 3 4 O 30 42 Match the steps of protein extraction with their specific purpos
Posted: Mon Jul 11, 2022 2:12 pm
by answerhappygod
- Ige 1 2 25 5 1 Gfp Is Approximately Kd 3 4 O 30 42 Match The Steps Of Protein Extraction With Their Specific Purpos 1 (32.07 KiB) Viewed 50 times
ige 1: 2- 25 5 1- GFP is approximately__kD. 3 4 O 30 42 Match the steps of protein extraction with their specific purpose. 27 063 Submit Quie Lysozyme solution 2 of 3
questions saved Resuspend the pellet in TE buffer TE buffer to hydrophobic column Binding buffer Wash Buffer 1. Contains EDTA which is a chelating agent binding to the positive charges in the suspension 2. Contains an enzyme to breakdown the cell wall and release intracellular GFP 3. Changes the charge of the protein to allow the GFP with bind to the column 4. Aids in removing any proteins that have different binding properties than GFP 5. Elutes the GFP from the column