Answer Happy

1. Fill in the outline noting the major differences in each step of transcription for prokaryotes and eukaryotes. Word bank: termination, elongation, initiation, Pribnow box, -10, -20, -35 consensus sequence, -25, -5-, -80,-90, TATA Box, CAAT, GC-rich box, TAAT, AC-rich box, TFIIB, TFIID, extrinsic termination, intrinsic termination, hairpin, loop, dissociate, bind, sigma-dependent, sigma, pi, rho-dependent, rho, polymerase, helicase, DNA, RNA, pre- translational modifications, post-translational modifications, 3' cap, 5' cap, Poly C Tail, Poly A Tail, intron splicing, DNAse, RNAse, polymerase I, polymerase II, polymerase III, Tornado model, Torpedo model, splicing model *some words may be used more than once and some words are not used at all. a. Step 1 - Prokaryotic Initiation: i. The consensus sequences are named and , and are located at respectively. ii. Attachment of a consensus sequences. b. Step 1 Eukaryotic Initiation: i. The consensus sequences are named and to the recognizes these ii. d. Step 2 - Eukaryotic Elongation. and , respectively. , a multi-subunit protein complex, binds to the consensus sequence. c. Step 2 - Prokaryotic Elongation. i. How many polymerases are needed and what is/are they called? and e. Step 3 - Prokaryotic Termination: i. i. How many polymerases are needed and what is/are they called? and are located at factor. This activates between is based on a termination sequence containing inverted repeats and a string of adenines to create a A series of U's follow this structure, causing the polymerase to termination and RNA. relies on the to break H bonds
f. Step 3 Eukaryotic Termination: i. before termination. These include _, and Termination then occurs via a special occur that digests the leftover transcript which it until it has reached the then dissociates from the DNA. This is called the