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marks 764-780 763 ence 366-692 ence 327-344 249-293 - ence 158-217 140-157 26-72 1171-2250 3684 4445-5302 5434-5889 b(+)
Posted: Sun Jul 10, 2022 4:19 pm
by answerhappygod
- Marks 764 780 763 Ence 366 692 Ence 327 344 249 293 Ence 158 217 140 157 26 72 1171 2250 3684 4445 5302 5434 5889 B 1 (57.54 KiB) Viewed 41 times
- Marks 764 780 763 Ence 366 692 Ence 327 344 249 293 Ence 158 217 140 157 26 72 1171 2250 3684 4445 5302 5434 5889 B 2 (17.45 KiB) Viewed 41 times
marks 764-780 763 ence 366-692 ence 327-344 249-293 - ence 158-217 140-157 26-72 1171-2250 3684 4445-5302 5434-5889 b(+) and PET-32c(+) 32a(+) (shown) with ons: pET-32b(+) is a act 1bp from each site 8. PET-32c(+) is a Ibp to each site 8 except for EcoR V. 17 promoter Scal(4995) Phu Hands) Pst (4760) Bsa l(4576) Eam1105 (4357) AlwN (4038) Dra Ill(5658) 4445-5302) Msc1 Xbal BspLU11 (3622) Sap (3506) - Bst1107 10393) TH191 19967). BspG (3148) lac operator TAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAA On (3684) 11 origin (5434-5889) Bou11021 PET-32a(+) (5900bp) Bpu1102 (80) S-Tag primer #69945-3 Ball Kon TAACTTTAAGAAGGAGA GGTATGAAAGAAACCGCTGCTOETAAATTCGAACGCCASCACATGGACAGECCAGATETOGGTACCGACGACGACGACAAS GlyMetLysGluThrAlaA10AlaysPheGluArginistetAspSerProAspleuGlyThrAspAspAspAspiys rbs His-Tag LysProGluArglysLeuser TrpleuleuProPraLeuserAnAnEnd Trx Tag His-Tag TATACATATGAGE 315bp CTGGCCGGTFCTOGTTCTGGCCATATGCACCATCATCATCATCATTCTTCTGGTCTGGTGCCACGEGGTTCT 10500 LeuAloGlySerGlySerGlyMetHisKiH SHISHISHisSerSerGlyLeuValProArglyser Meter S-Tag Nap V AloMetAlolleber AspProAsnserer SerValApplysLeuAloAlokloLeuGluhishishishisishind Bgl (241) -Kpn 1(238) -Nsp V(268) Msc (351) Rsr Il(589) 1XA (366-692) T7 terminator T7 terminator primer #69337-3 PET-32a-c(+) cloning/expression region Psp5112628) DET-32a(+) Eagl Aval Nool EcoR V BamHI EcoRI Sac Sall Hind Ill Not I Xhol GCCATOGCYGATATCGGATCEGAATTCGAGCTCCGTCGACAAGCTTGCOGECOCACTEGAGCACCACCACCACCACCACTGAGATECOGETOETAA AloMetAloAsplleGlySerGluPheGiuleuArgArgGInAlaCysGiyArgThrArgAloProProProProPraLeuArglierGlycysEnd CCCATGGCGATATCGEATCEGAATTCGAGETECGTCGACAAGETTGEGGCCGCACTCGAGCACCACCACCACCACCACTGAGATCEGGETOCTAA Ava (158) Xho (158) Eag (166) Not (166) Hind Ill(173) Sal (179) Sac (190) ECOR (192) BamH (198) EcoR V(206) Nco (212) thrombin enterokinase .Xba I(729) AloMetályTyrLeutrolleAralleArgAloProSer ThrSerLeuAroProtisSerSerTheThe ThrTheTheThralulleArdeuleuTh PshA (2366) CAAAGCCCGAAAGGAAGCTGAGTTOGETOCTOCCACCGETGASCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGOGTCTTGAGGGGTTTTTT SgrA (840) lacl (1171-2250) Sph 1996) EcoN (1056) GCCATOGGATATCTGTOGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGASCACCACCACCACCACCACTOAGATCCGGCTGCTAA PET-32c(+) ApaB (1205) Miu (1521) Bel (1535) BstE (1702) Bmg (1730) Apa (1732) BssH (1932) Hpa (2027) PET-32b(+)
4.1. How many fragments will form if pET32a(+) is digested simultaneously with Sacl and Bcll. [1] 4.2. Write down the base pairs that result at the 5' and 3' ends of the shortest fragment [3] 4.3. Restriction enzymes often recognize palindromic sequences. What is a palindromic sequence? [2] 4.4. Give three restriction enzymes found on the pET32(a+) multiple cloning site. [3]