Exercise 2: Genetic Mutations In this activity, you will create different mutations in the same nucleic acid sequence an
Posted: Sun Jul 10, 2022 4:18 pm
Exercise 2: Genetic Mutations In this activity, you will createdifferent mutations in the same nucleic acid sequence and examinehow they affect the resulting protein. (Note: to save time, youwill introduce mutations in the RNA transcript rather than startingwith the DNA and transcribing first, then translating. Keep in mindthat any mutations in the RNA would have originated in theDNA.)
7. Translate the following sequence. (Remember, unless otherwisenoted, a nucleic acid sequence is written from 5’ to 3’, so you donot need to reverse these sequences like you did with the ones youtranscribed from the DNA.) AUG AGU GAU GUU GAG AGA CAU UUA GAU GUCUUU AAG
8. Create a point mutation in this sequence. (That is, change asingle nucleotide to a different one). Highlight the singlenucleotide that you changed. DO NOT highlight the entire codon.
AUG AGU GAU GUU GAG AGA CAU UUA GAU GUC UUU AAG
9. Translate your mutated transcript from #8. Highlight theaffected amino acid.
10.Did your point mutation cause a silent mutation, a missensemutation, or a nonsense mutation? Explain your answer.
11.Now create a frameshift mutation by inserting one base (oneletter, not a whole codon) somewhere near the beginning or middleof the sequence. Highlight the inserted bases. AUG AGU GAU GUU GAGAGA CAU UUA GAU GUC UUU AAG
12.Translate your mutated transcript from #11.
13.Create a frameshift mutation by deleting one base somewherenear the beginning or middle of the sequence. Replace each deletedbase with a hyphen (-). AUG AGU GAU GUU GAG AGA CAU UUA GAU GUC UUUAAG
14. Translate your mutated transcript from #13. (Remove thehyphens first!)
15.In the spaces below, enter the amino acid sequences from #7,9, 12, and 14. Use different highlight or text colors to indicatewhere each mutated sequence differs from the normal sequence.
7 (normal):
9 (point mutation):
12 (insertion):
14 (deletion):
16. Describe any differences you observe between the results ofa point mutation compared to a frameshift mutation. That is, howdoes a point mutation affect the resulting protein differently thanan insertion or deletion? (Keep in mind that some – but not all –point mutations lead to a silent mutation that does not cause anyamino acid change.)
7. Translate the following sequence. (Remember, unless otherwisenoted, a nucleic acid sequence is written from 5’ to 3’, so you donot need to reverse these sequences like you did with the ones youtranscribed from the DNA.) AUG AGU GAU GUU GAG AGA CAU UUA GAU GUCUUU AAG
8. Create a point mutation in this sequence. (That is, change asingle nucleotide to a different one). Highlight the singlenucleotide that you changed. DO NOT highlight the entire codon.
AUG AGU GAU GUU GAG AGA CAU UUA GAU GUC UUU AAG
9. Translate your mutated transcript from #8. Highlight theaffected amino acid.
10.Did your point mutation cause a silent mutation, a missensemutation, or a nonsense mutation? Explain your answer.
11.Now create a frameshift mutation by inserting one base (oneletter, not a whole codon) somewhere near the beginning or middleof the sequence. Highlight the inserted bases. AUG AGU GAU GUU GAGAGA CAU UUA GAU GUC UUU AAG
12.Translate your mutated transcript from #11.
13.Create a frameshift mutation by deleting one base somewherenear the beginning or middle of the sequence. Replace each deletedbase with a hyphen (-). AUG AGU GAU GUU GAG AGA CAU UUA GAU GUC UUUAAG
14. Translate your mutated transcript from #13. (Remove thehyphens first!)
15.In the spaces below, enter the amino acid sequences from #7,9, 12, and 14. Use different highlight or text colors to indicatewhere each mutated sequence differs from the normal sequence.
7 (normal):
9 (point mutation):
12 (insertion):
14 (deletion):
16. Describe any differences you observe between the results ofa point mutation compared to a frameshift mutation. That is, howdoes a point mutation affect the resulting protein differently thanan insertion or deletion? (Keep in mind that some – but not all –point mutations lead to a silent mutation that does not cause anyamino acid change.)