Answer Happy

You are studying protein Con 12. To find out its location and trafficking, you have treated cells with two chemicals (Figure 2, lanes 2-3). After performing SDS-PAGE and immunoblotting with an antibody against Con12, you discover different forms of the Con12 protein. Examine the Con12 immunoblot in Figure 2 Con12. Briefly explain: 1) Why does adding endoglycosidase F (see Figure 2: Hint for lane 2) result in only one, lower form in lane 2? [4 min] 2) Why does stopping protein production with cycloheximide make the lower form disappear in lane 3? [5 min] 3