Answer Happy

(a) Discuss in detail about molecular diffusion process. (b) Dynamic method is one the methods to measure k₁, (oxygen mass transfer coefficient) (4) (6) in bioprocessing. Discuss this method with an example. (c) E. coli is being grown in a 52 litres capacity standard aerobic fermentation in a pilot (10) plant experimentation. The fermentation broth is agitated with a turbine impeller. The dimension of the bioreactor and the liquid height in the vessel are as per standard dimensions. Air is being blown into the fermenter at 12 1/s. The gas hold-up is estimated to be 17%. The turbine impeller is operated with 0.1hp motor; out of which only 10% of the power is being used for agitating the impeller. Estimate the mass transfer rate (ka).