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Compare the functioning of tropomyosin to troponin in skeletal muscle contraction. Selected answer will be automatically saved. For keyboard navigation, press up/down arrow keys to select an answer. b d Tropomyosin binds to Ca2+, allowing troponin to catalyze the formation of a cross-bridge between actin and myosin. One portion of troponin binds Ca2+, moving tropomyosin out of the way for actin and myosin to form a cross-bridge. Troponin stores Ca2+, which is released during skeletal muscle contraction, tropomyosin ensures that the actin and myosin filaments are properly aligned to each other. Tropomyosin stores Ca2+, which is released during skeletal muscle contraction, troponin ensures that the actin and myosin filaments are properly aligned to each other.