Answer Happy

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5. Determine whether the proteins below (we studied these in class) perform uniport, symport, or antiport function. Indicate whether they carry out active or passive transport, and where in the animal cell these proteins are typically located. (2 points each, 12 points total) Na+pump a) uniport/symport/antiport? b) active/passive transport? c) location: pyruvate transporter a) uniport/symport/antiport? b) active/passive transport? c) location:
7. You have isolated a yeast protein that normally resides in the lumen of the endoplasmic reticulum, and found that this protein has a specific sequence of four amino acids at its carboxy terminus that you suspect functions as an ER retention signal. A. What experiment would demonstrate that this sequence is necessary for retaining this protein in the ER? Describe the experiment in one sentence, and indicate the expected outcome in the second sentence. (2 points each; 4 total) Experiment: Expected outcome: B. What experiment would demonstrate that this sequence is sufficient for retaining this protein in the ER? Describe the experiment in one sentence, and indicate the expected outcome in the second sentence. (2 points each; 4 total) Experiment: Expected outcome: